Chromatin Immunoprecipitation:Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Flow Cytometry:Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Magnetic Resonance Imaging:Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Biomarker Discovery:Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Article Title: Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Below is a summary of commercially available antibodies used in this study: or as cited in the Materials and methods section: FOXA2 R&D Systems AF2400 1:500 HHEX R&D Systems MAB83771 Clone#2018B 1:100 TBX3 abcam ab99302 1:500 PDX1 R&D Systems AF2419 1:500 SOX9 Millipore AB5535 1:300 AFP R&D Systems MAB1369 Clone#189506 1:500 ALB R&D Systems AF3329 1:500 NKX6-2 abcam ab58708 1:100 ROBO2 Cell Signaling Technology 45568S 1:100 GP2 MBL INTERNATIONAL D277-5 3G7-H9 1:100 INS Dako a0564 1:1000 H3K4me1 abcam ab8895 1:50 H3K27ac abcam ab4729 1:50 FOXA1 abcam ab170933 EPR10881 1:50 CD184-PECy7 BDBiosciences 560669 12G5 (RUO) 1:100 CD117-APC BDBiosciences 561118 YB5.B8 (RUO) 1:100 Alexa fluor 488 anti-goat Thermo Fisher A11055 1:800 Alexa fluor 568 anti-goat Thermo Fisher A11057 1:800 Alexa fluor 647 anti-goat Thermo Fisher A21447 1:800 Alexa fluor 488 anti-mouse Thermo Fisher A21202 1:800 Alexa fluor 568 anti-mouse Thermo Fisher A10037 1:800 Alexa fluor 488 anti-rabbit Thermo Fisher A32790 1:800 Alexa fluor 568 anti-rabbit Thermo Fisher A10042 1:800 Alexa fluor 647 anti-rabbit Thermo Fisher A32795 1:500 Validation Antibodies were validated as noted on manufacturer's websites as following: - FOXA2 R&D Systems AF2400 1:500; https://www.rndsystems.com/products/human-hnf-3beta-foxa2-antibody_af2400 - HHEX R&D Systems MAB83771 Clone#2018B 1:100; https://www.rndsystems.com/products/human-mouse-rat-hhexantibody-2018b_mab83771 - TBX3 abcam ab99302 1:500; https://www.abcam.com/tbx3-antibody-ab99302.html - PDX1 R&D Systems AF2419 1:500; https://www.rndsystems.com/products/human-pdx-1-ipf1-antibody_af2419 - SOX9 Millipore AB5535 1:300; https://www.merckmillipore.com/DK/en/product/Anti-Sox9,MM_NF-AB5535-25UG? ..
Control:Article Title: Spatial Single Cell Analysis of Proteins in 2D Human Gastruloids Using Iterative Immunofluorescence.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Table 1 Primary Antibodies Antigen Manufacturer Catalog number Dilution β-catenin BD Biosciences BDB610154 1/200 CDX2 BioGenex MU392A-5UC 1/50 EOMES R&D Systems MAB6166 1/200 FOXA2 R&D Systems AF2400 1/500 GATA3 SantaCruz Biotechnology sc-268 1/50 HAND1 R&D Systems AF3168 1/200 ISL1 DSHB 39.4D5 1/200 LEF1 Cell Signaling Tech C12A5 1/100 MIXL1 ABclonal A17223 1/150 NANOG R&D Systems AF1997 1/100 OCT3/4 BD Biosciences 611202 1/400 OTX2 R&D Systems AF1979 1/150 pAKT (Ser473) Cell Signaling Tech 4060 1/200 pERK Cell Signaling Tech 9101S 1/100 PRDM1 SantaCruz Biotechnology sc-47732 1/25 SMAD1 Cell Signaling Tech 6944T 1/100 SMAD2 Cell Signaling Tech 5339T 1/50 pSMAD1/5/9 Cell Signaling Tech 13820S 1/50 SMAD2/3 BD Biosciences 610843 1/50 SNAI1 R&D Systems AF3639 1/100 SOX17 R&D Systems AF1924 1/1000 SOX2 Cell Signaling Tech 3579S 1/200 TBX6 R&D Systems AF4744 1/100 TBXT R&D Systems AF2085 1/300 TFAP2C SantaCruz Biotechnology sc-12762 1/150 YAP Cell Signaling Tech 14074T 1/100 Table 2 Secondary Antibodies Antigen Manufacturer Cat. no. Dilution Donkey anti-mouse Alexa Fluor 488 Thermo Fisher Scientific A21202 1/500 Donkey anti-rabbit Alexa Fluor 555 Thermo Fisher Scientific A31572 1/500 Donkey anti-goat Alexa Fluor 647 Thermo Fisher Scientific A21447 1/500 Donkey anti-rabbit Alexa Fluor 647 Thermo Fisher Scientific A31573 1/500 Donkey anti-goat Alexa Fluor 555 Thermo Fisher Scientific A21432 1/500 Donkey anti-rat Alexa Fluor 555 Abcam ab150154 1/500 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific A21206 1/500 quality control by repeating one of the first-round stains in a later round and calculating how the single cell intensities of this stain correlate between rounds. ..
Single Cell:Article Title: Spatial Single Cell Analysis of Proteins in 2D Human Gastruloids Using Iterative Immunofluorescence.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Table 1 Primary Antibodies Antigen Manufacturer Catalog number Dilution β-catenin BD Biosciences BDB610154 1/200 CDX2 BioGenex MU392A-5UC 1/50 EOMES R&D Systems MAB6166 1/200 FOXA2 R&D Systems AF2400 1/500 GATA3 SantaCruz Biotechnology sc-268 1/50 HAND1 R&D Systems AF3168 1/200 ISL1 DSHB 39.4D5 1/200 LEF1 Cell Signaling Tech C12A5 1/100 MIXL1 ABclonal A17223 1/150 NANOG R&D Systems AF1997 1/100 OCT3/4 BD Biosciences 611202 1/400 OTX2 R&D Systems AF1979 1/150 pAKT (Ser473) Cell Signaling Tech 4060 1/200 pERK Cell Signaling Tech 9101S 1/100 PRDM1 SantaCruz Biotechnology sc-47732 1/25 SMAD1 Cell Signaling Tech 6944T 1/100 SMAD2 Cell Signaling Tech 5339T 1/50 pSMAD1/5/9 Cell Signaling Tech 13820S 1/50 SMAD2/3 BD Biosciences 610843 1/50 SNAI1 R&D Systems AF3639 1/100 SOX17 R&D Systems AF1924 1/1000 SOX2 Cell Signaling Tech 3579S 1/200 TBX6 R&D Systems AF4744 1/100 TBXT R&D Systems AF2085 1/300 TFAP2C SantaCruz Biotechnology sc-12762 1/150 YAP Cell Signaling Tech 14074T 1/100 Table 2 Secondary Antibodies Antigen Manufacturer Cat. no. Dilution Donkey anti-mouse Alexa Fluor 488 Thermo Fisher Scientific A21202 1/500 Donkey anti-rabbit Alexa Fluor 555 Thermo Fisher Scientific A31572 1/500 Donkey anti-goat Alexa Fluor 647 Thermo Fisher Scientific A21447 1/500 Donkey anti-rabbit Alexa Fluor 647 Thermo Fisher Scientific A31573 1/500 Donkey anti-goat Alexa Fluor 555 Thermo Fisher Scientific A21432 1/500 Donkey anti-rat Alexa Fluor 555 Abcam ab150154 1/500 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific A21206 1/500 quality control by repeating one of the first-round stains in a later round and calculating how the single cell intensities of this stain correlate between rounds. ..
Staining:Article Title: Spatial Single Cell Analysis of Proteins in 2D Human Gastruloids Using Iterative Immunofluorescence.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Table 1 Primary Antibodies Antigen Manufacturer Catalog number Dilution β-catenin BD Biosciences BDB610154 1/200 CDX2 BioGenex MU392A-5UC 1/50 EOMES R&D Systems MAB6166 1/200 FOXA2 R&D Systems AF2400 1/500 GATA3 SantaCruz Biotechnology sc-268 1/50 HAND1 R&D Systems AF3168 1/200 ISL1 DSHB 39.4D5 1/200 LEF1 Cell Signaling Tech C12A5 1/100 MIXL1 ABclonal A17223 1/150 NANOG R&D Systems AF1997 1/100 OCT3/4 BD Biosciences 611202 1/400 OTX2 R&D Systems AF1979 1/150 pAKT (Ser473) Cell Signaling Tech 4060 1/200 pERK Cell Signaling Tech 9101S 1/100 PRDM1 SantaCruz Biotechnology sc-47732 1/25 SMAD1 Cell Signaling Tech 6944T 1/100 SMAD2 Cell Signaling Tech 5339T 1/50 pSMAD1/5/9 Cell Signaling Tech 13820S 1/50 SMAD2/3 BD Biosciences 610843 1/50 SNAI1 R&D Systems AF3639 1/100 SOX17 R&D Systems AF1924 1/1000 SOX2 Cell Signaling Tech 3579S 1/200 TBX6 R&D Systems AF4744 1/100 TBXT R&D Systems AF2085 1/300 TFAP2C SantaCruz Biotechnology sc-12762 1/150 YAP Cell Signaling Tech 14074T 1/100 Table 2 Secondary Antibodies Antigen Manufacturer Cat. no. Dilution Donkey anti-mouse Alexa Fluor 488 Thermo Fisher Scientific A21202 1/500 Donkey anti-rabbit Alexa Fluor 555 Thermo Fisher Scientific A31572 1/500 Donkey anti-goat Alexa Fluor 647 Thermo Fisher Scientific A21447 1/500 Donkey anti-rabbit Alexa Fluor 647 Thermo Fisher Scientific A31573 1/500 Donkey anti-goat Alexa Fluor 555 Thermo Fisher Scientific A21432 1/500 Donkey anti-rat Alexa Fluor 555 Abcam ab150154 1/500 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific A21206 1/500 quality control by repeating one of the first-round stains in a later round and calculating how the single cell intensities of this stain correlate between rounds. ..
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